Green Fluorescent Protein as a Marker for Gene Expression

Martin Chalfie, Yuan Tu, Ghia Euskirchen, William W. Ward, Douglas C. Prasher

DSEID: DSEID-007-4642115
DOI: 10.1126/science.8303295
Journal: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1994-2-11
Status: temporarily_unreachable

Abstract

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic ( Escherichia coli ) or eukaryotic ( Caenorhabditis elegans ) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

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Metadata

Title: Green Fluorescent Protein as a Marker for Gene Expression
Delta ID: DSEID-007-4642115
Authors: Martin Chalfie, Yuan Tu, Ghia Euskirchen, William W. Ward, Douglas C. Prasher
Abstract source: crossref
Source URL: https://doi.org/10.1126/science.8303295
Access: closed_or_uncertain
Licence: unknown
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