DNA sequencing with chain-terminating inhibitors

F. Sanger, S. Nicklen, A. R. Coulson

DSEID: DSEID-007-2502262
DOI: 10.1073/pnas.74.12.5463
Journal: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1977-12
Status: available

Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

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Metadata

Title: DNA sequencing with chain-terminating inhibitors
Delta ID: DSEID-007-2502262
Authors: F. Sanger, S. Nicklen, A. R. Coulson
Abstract source: crossref
Source URL: https://epub.uni-regensburg.de/18678/1/sumper1.pdf
Access: open_publisher
Licence: unknown
PDF SHA-256: cf287a9778362ad175d5b4d7813e9cd6ecd5ab21466481ce50a9a6bddc40f5ad
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